Xylene or toluene must be used after cedarwood oil.

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Multiple Choice

Xylene or toluene must be used after cedarwood oil.

Explanation:
After cedarwood oil is used as the clearing step, a transitional solvent is needed to make the tissue ready for paraffin infiltration. Cedarwood oil is not compatible with paraffin, so xylene or toluene is used to remove the cedarwood oil and replace it with a solvent that mixes with paraffin. This bridging step is essential for proper paraffin infiltration and embedding; skipping it can leave residue or hinder infiltration, compromising tissue morphology. Therefore, using xylene or toluene after cedarwood oil is required.

After cedarwood oil is used as the clearing step, a transitional solvent is needed to make the tissue ready for paraffin infiltration. Cedarwood oil is not compatible with paraffin, so xylene or toluene is used to remove the cedarwood oil and replace it with a solvent that mixes with paraffin. This bridging step is essential for proper paraffin infiltration and embedding; skipping it can leave residue or hinder infiltration, compromising tissue morphology. Therefore, using xylene or toluene after cedarwood oil is required.

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