Which tissue is used as the control slide for Cresyl Echt Violet?

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Multiple Choice

Which tissue is used as the control slide for Cresyl Echt Violet?

Explanation:
Cresyl violet is a Nissl stain that binds to RNA in the rough endoplasmic reticulum of neuronal cell bodies, highlighting Nissl substance. Because grey matter is rich in neuron cell bodies and their Nissl bodies, it provides a clear reference pattern to verify that the stain is working and producing the expected Purple/blue coloration of neuronal cell bodies. Tissues like spleen or liver, small intestine, or aorta/skin lack the dense population of neuron cell bodies or the Nissl substance needed for this stain, so they don’t serve as reliable controls. Therefore, grey matter is the best choice for a control slide.

Cresyl violet is a Nissl stain that binds to RNA in the rough endoplasmic reticulum of neuronal cell bodies, highlighting Nissl substance. Because grey matter is rich in neuron cell bodies and their Nissl bodies, it provides a clear reference pattern to verify that the stain is working and producing the expected Purple/blue coloration of neuronal cell bodies. Tissues like spleen or liver, small intestine, or aorta/skin lack the dense population of neuron cell bodies or the Nissl substance needed for this stain, so they don’t serve as reliable controls. Therefore, grey matter is the best choice for a control slide.

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