Which technique could also be used to demonstrate the substance stained red in the image?

• A. Sudan Black B
• B. Hematoxylin
• C. Eosin
• D. Methylene Blue

Answer: (A)

Staining lipids relies on fat‑soluble dyes that bind to the lipid components in tissues. The red-stained substance in the image is consistent with a lipid-containing material, so using an additional lipid stain can confirm its nature. Sudan Black B is a fat‑soluble dye that binds neutral fats and phospholipids, allowing lipid‑rich areas to be demonstrated even if the color changes with the stain. Hematoxylin and eosin target nuclei and cytoplasmic/eosinophilic structures, respectively, and aren’t specific for lipids. Methylene Blue is a general basic stain for cell details, not a lipids-specific stain. Therefore, Sudan Black B is the appropriate alternative to demonstrate the same lipid-containing substance.