Which step uses heat in the schedule?

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Multiple Choice

Which step uses heat in the schedule?

Explanation:
Heat is used during the paraffin infiltration/embedding step. Paraffin must be melted to a liquid so it can permeate the tissue and replace the previous dehydrants, and the embedding station keeps the wax molten (around 60°C) to allow complete infiltration and later solidification into a block. The other steps—fixation with formalin, dehydration with 95% alcohol, and clearing with xylene—are performed at ambient or controlled temperatures and do not rely on sustained heating in the same way.

Heat is used during the paraffin infiltration/embedding step. Paraffin must be melted to a liquid so it can permeate the tissue and replace the previous dehydrants, and the embedding station keeps the wax molten (around 60°C) to allow complete infiltration and later solidification into a block. The other steps—fixation with formalin, dehydration with 95% alcohol, and clearing with xylene—are performed at ambient or controlled temperatures and do not rely on sustained heating in the same way.

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