Which step is not part of routine processing?

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Multiple Choice

Which step is not part of routine processing?

Explanation:
In paraffin histology, the steps of routine processing are aimed at preserving and hardening the tissue so it can be cut into thin sections. Fixation preserves cellular structure; dehydration removes water; clearing makes the tissue miscible with paraffin; then infiltration with paraffin and embedding provide the solid block needed for slicing. Sectioning, performed with a microtome to produce thin slices from the embedded block, occurs after embedding and is part of slide preparation, not the processing itself. Therefore, sectioning is not part of routine processing.

In paraffin histology, the steps of routine processing are aimed at preserving and hardening the tissue so it can be cut into thin sections. Fixation preserves cellular structure; dehydration removes water; clearing makes the tissue miscible with paraffin; then infiltration with paraffin and embedding provide the solid block needed for slicing. Sectioning, performed with a microtome to produce thin slices from the embedded block, occurs after embedding and is part of slide preparation, not the processing itself. Therefore, sectioning is not part of routine processing.

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