Which statement about Celestine Blue is true?

• A. It may be substituted for iron hematoxylin
• B. It stains cytoplasm intensely
• C. It is used as the primary nuclear stain
• D. It stains lipids

Answer: (A)

Celestine blue works as a nuclear stain by binding to acidic nuclear components like DNA and RNA, giving nuclei a blue color. Because of this, it can be used in place of iron hematoxylin in certain staining protocols to visualize nuclei, offering a substitute approach rather than relying on iron hematoxylin. It isn’t used to stain cytoplasm intensely, it’s not the typical primary nuclear stain in standard H&E procedures, and it doesn’t stain lipids. So the true statement is that Celestine blue may substitute for iron hematoxylin.