Which staining sequence is used to detect glycogen (before/after did digestion)?

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Multiple Choice

Which staining sequence is used to detect glycogen (before/after did digestion)?

Explanation:
Glycogen detection relies on its susceptibility to diastase digestion. The PAS stain reveals carbohydrates, including glycogen, but to confirm glycogen specifically you perform diastase digestion before the PAS reaction. If the tissue loses PAS staining after diastase treatment, that indicates the original PAS-positive material was glycogen, since glycogen is destroyed by the enzyme. The sequence that administers diastase first, then periodic acid and Schiff reagent, is the diastase-PAS approach used to demonstrate glycogen. In contrast, a plain PAS stain would stain glycogen but isn’t specific; Alcian Blue targets acidic mucopolysaccharides, and Congo Red identifies amyloid.

Glycogen detection relies on its susceptibility to diastase digestion. The PAS stain reveals carbohydrates, including glycogen, but to confirm glycogen specifically you perform diastase digestion before the PAS reaction. If the tissue loses PAS staining after diastase treatment, that indicates the original PAS-positive material was glycogen, since glycogen is destroyed by the enzyme. The sequence that administers diastase first, then periodic acid and Schiff reagent, is the diastase-PAS approach used to demonstrate glycogen. In contrast, a plain PAS stain would stain glycogen but isn’t specific; Alcian Blue targets acidic mucopolysaccharides, and Congo Red identifies amyloid.

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