Which staining procedure uses xylene-peanut oil for deparaffinization?

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Multiple Choice

Which staining procedure uses xylene-peanut oil for deparaffinization?

Explanation:
Specialty deparaffinization is used for certain delicate, lipid-rich organisms. The Fite–Fast acid-fast staining method is designed to visualize Mycobacterium leprae in tissue by deparaffinizing with a mixture of xylene and peanut oil. The peanut oil helps preserve the tissue lipids and the waxy mycobacterial cell wall during solvent extraction, allowing the primary stain to be retained and the organisms to be seen after the milder decolorization step. Other stains listed rely on standard xylene deparaffinization (or different staining principles) and do not use the xylene-peanut oil combination.

Specialty deparaffinization is used for certain delicate, lipid-rich organisms. The Fite–Fast acid-fast staining method is designed to visualize Mycobacterium leprae in tissue by deparaffinizing with a mixture of xylene and peanut oil. The peanut oil helps preserve the tissue lipids and the waxy mycobacterial cell wall during solvent extraction, allowing the primary stain to be retained and the organisms to be seen after the milder decolorization step. Other stains listed rely on standard xylene deparaffinization (or different staining principles) and do not use the xylene-peanut oil combination.

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