Which staining method is positive for glycogen and uses Bauer-feulgen?

• A. Bauer-feulgen
• B. PAS
• C. Best carmine
• D. Iodine method

Answer: (A)

Glycogen detection in tissue relies on staining methods that reveal glucose polymers inside cells. The Bauer–Feulgen method is a modification of Schiff-based Feulgen chemistry tailored for polysaccharides like glycogen. In this approach, carbohydrate residues are treated to generate aldehyde groups, which then react with Schiff reagent to produce a magenta color where glycogen is present. That specific chemistry is what makes glycogen stand out with this method. Other glycogen stains use different reactions—for example, some rely on iodine complex formation or carmine binding—so they aren’t based on the Bauer–Feulgen reaction. Therefore, the staining method that is positive for glycogen and uses Bauer–Feulgen is the Bauer–Feulgen staining technique.