Which stain is used to demonstrate axons and components of the peripheral nervous system?

• A. Bodian
• B. Holzer
• C. Bielschowsky
• D. Cresyl echt Violet

Answer: (A)

Visualizing axons in the peripheral nervous system relies on a stain that specifically impregnates axons with silver so they stand out against a light background. The Bodian stain is a silver impregnation method that highlights axons and nerve fibers, giving dark markings on a pale backdrop. This makes it especially useful for examining peripheral nerves, where you want to see individual axons, their integrity, and any degenerative changes or regeneration.

Other stains don’t target axons in the same way. Cresyl echt violet stains neuronal cell bodies (Nissl substance) rather than axons. Bielschowsky can reveal axons and neurofibrils but is more broadly used and not as specifically suited to peripheral nerve components. Holzer stain highlights myelin rather than the axons themselves.