Which stain demonstrates RNA?

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Multiple Choice

Which stain demonstrates RNA?

Explanation:
RNA is demonstrated using a differential nucleic acid stain that separates DNA from RNA. The methyl green-pyronin combination works this way: methyl green binds DNA, giving it green color, while pyronin Y binds RNA, coloring it red/pink. This makes RNA-rich areas, especially cytoplasmic ribosomal RNA and the nucleolus, stand out against the green-stained DNA. The other stains don’t specifically highlight RNA in a distinct color: hematoxylin mainly stains nuclei (DNA) blue-purple, eosin stains cytoplasm and extracellular proteins pink, and PAS highlights carbohydrates like glycogen. So, the stain that best shows RNA is the methyl green-pyronin combination.

RNA is demonstrated using a differential nucleic acid stain that separates DNA from RNA. The methyl green-pyronin combination works this way: methyl green binds DNA, giving it green color, while pyronin Y binds RNA, coloring it red/pink. This makes RNA-rich areas, especially cytoplasmic ribosomal RNA and the nucleolus, stand out against the green-stained DNA. The other stains don’t specifically highlight RNA in a distinct color: hematoxylin mainly stains nuclei (DNA) blue-purple, eosin stains cytoplasm and extracellular proteins pink, and PAS highlights carbohydrates like glycogen. So, the stain that best shows RNA is the methyl green-pyronin combination.

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