Which stain demonstrates Nissl substance in neurons?

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Multiple Choice

Which stain demonstrates Nissl substance in neurons?

Explanation:
Nissl substance are the RNA-rich parts of the rough endoplasmic reticulum in the neuron’s soma, which appear as basophilic granules. A basic dye that binds RNA highlights these structures strongly, so cresyl violet stains the Nissl bodies blue‑violet and makes the neuronal cell body stand out from surrounding tissue. This staining is particularly useful for examining neuronal morphology and for noticing chromatolysis, a sign of neuronal injury where the Nissl substance disperses. The other stains mentioned have different targets: Congo red binds amyloid deposits, Giemsa is used for blood smears and certain parasites, and Alcian blue stains acidic mucopolysaccharides.

Nissl substance are the RNA-rich parts of the rough endoplasmic reticulum in the neuron’s soma, which appear as basophilic granules. A basic dye that binds RNA highlights these structures strongly, so cresyl violet stains the Nissl bodies blue‑violet and makes the neuronal cell body stand out from surrounding tissue. This staining is particularly useful for examining neuronal morphology and for noticing chromatolysis, a sign of neuronal injury where the Nissl substance disperses.

The other stains mentioned have different targets: Congo red binds amyloid deposits, Giemsa is used for blood smears and certain parasites, and Alcian blue stains acidic mucopolysaccharides.

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