Which solvent is used to prevent lipid loss during fat staining with Oil Red O and Sudan Black B?

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Multiple Choice

Which solvent is used to prevent lipid loss during fat staining with Oil Red O and Sudan Black B?

Explanation:
Preserving lipids during fat staining requires a solvent that won’t aggressively extract fats from the tissue. Propylene glycol acts as a mild, lipid-friendly carrier that dissolves Oil Red O and Sudan Black B well while keeping lipids in place during staining and rinsing. In contrast, ethanol, acetone, and especially xylene can extract or remove lipids, leading to loss of fat signal. Using propylene glycol therefore provides better preservation of lipid content while allowing effective staining with both Oil Red O and Sudan Black B.

Preserving lipids during fat staining requires a solvent that won’t aggressively extract fats from the tissue. Propylene glycol acts as a mild, lipid-friendly carrier that dissolves Oil Red O and Sudan Black B well while keeping lipids in place during staining and rinsing. In contrast, ethanol, acetone, and especially xylene can extract or remove lipids, leading to loss of fat signal. Using propylene glycol therefore provides better preservation of lipid content while allowing effective staining with both Oil Red O and Sudan Black B.

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