Which preventive measure would have avoided the problem shown in the image?

• A. Ensuring that both sections were embedded flat and at the same level
• B. Improper embedding
• C. Forceps metastasis
• D. Better cleaning of forceps between specimens

Answer: (A)

Ensuring both sections are embedded flat and at the same level keeps them in the same plane for sectioning. When tissues are placed at different heights in the mold, the microtome cuts through them at different depths, producing uneven or misregistered sections. The result on the slide can be partial tissue, one section appearing thicker, or an incomplete view of either tissue—the issue shown in the image. By making sure the top surfaces of both sections are level with the block face, you get uniform sections cut in the same plane, avoiding that artifact. The other options address different problems: improper embedding is a vague description of embedding quality, cross-contamination from forceps relates to instrument cleanliness rather than sectioning plane, and cleaning forceps between specimens would prevent transfer of material rather than prevent uneven embedding.