Which PAS-related approach uses enzymes to distinguish glycogen from other polysaccharides?

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Multiple Choice

Which PAS-related approach uses enzymes to distinguish glycogen from other polysaccharides?

Explanation:
Using diastase in a PAS staining protocol tests for glycogen by exploiting a specific enzymatic digestion. PAS stains carbohydrates magenta, but glycogen is digested by diastase (an amylase) into smaller sugars, which removes its PAS coloration. Other polysaccharides in tissue, such as mucopolysaccharides and glycoproteins, aren’t digested by diastase, so they remain PAS-positive after treatment. This makes it possible to tell glycogen apart from other PAS-positive substances by performing PAS with diastase (PAS-D). The other options don’t involve enzymatic digestion of glycogen, so they don’t provide this selective distinction.

Using diastase in a PAS staining protocol tests for glycogen by exploiting a specific enzymatic digestion. PAS stains carbohydrates magenta, but glycogen is digested by diastase (an amylase) into smaller sugars, which removes its PAS coloration. Other polysaccharides in tissue, such as mucopolysaccharides and glycoproteins, aren’t digested by diastase, so they remain PAS-positive after treatment. This makes it possible to tell glycogen apart from other PAS-positive substances by performing PAS with diastase (PAS-D). The other options don’t involve enzymatic digestion of glycogen, so they don’t provide this selective distinction.

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