Which fixatives are used for Verhoeff-van Gieson?

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Multiple Choice

Which fixatives are used for Verhoeff-van Gieson?

Explanation:
Elastic fibers are highlighted by Verhoeff-van Gieson when they are well preserved by the fixative. The fixative should maintain tissue architecture and elastin integrity without causing excessive dehydration or masking of the fibers. 10% neutral buffered formalin is a standard, widely compatible fixative that preserves elastin well for this stain. Zenker’s fixative, a mercuric chloride–based solution, also preserves elastic fibers effectively and is commonly used for elastic-stain procedures. Alcohol fixation, in contrast, tends to dehydrate and shrink tissue and can compromise elastin preservation and stain uptake. So, both 10% NBF and Zenker’s fixative are suitable for Verhoeff-van Gieson.

Elastic fibers are highlighted by Verhoeff-van Gieson when they are well preserved by the fixative. The fixative should maintain tissue architecture and elastin integrity without causing excessive dehydration or masking of the fibers. 10% neutral buffered formalin is a standard, widely compatible fixative that preserves elastin well for this stain. Zenker’s fixative, a mercuric chloride–based solution, also preserves elastic fibers effectively and is commonly used for elastic-stain procedures. Alcohol fixation, in contrast, tends to dehydrate and shrink tissue and can compromise elastin preservation and stain uptake. So, both 10% NBF and Zenker’s fixative are suitable for Verhoeff-van Gieson.

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