Which fixative cannot be used with Schiff's reagent?

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Multiple Choice

Which fixative cannot be used with Schiff's reagent?

Explanation:
Schiff's reagent in PAS staining detects aldehyde groups formed by oxidation of certain carbohydrates. Tissues fixed with glutaraldehyde undergo extensive cross-linking of proteins, which masks or blocks carbohydrate sites and prevents the formation of the reactive aldehydes needed for the Schiff reaction. Because of this masking effect, PAS staining is poor or negative with glutaraldehyde-fixed tissue. The other fixatives listed don’t mask those aldehyde sites to the same extent, so they are compatible with Schiff’s reagent, though some background or intensity differences may occur depending on the fixative.

Schiff's reagent in PAS staining detects aldehyde groups formed by oxidation of certain carbohydrates. Tissues fixed with glutaraldehyde undergo extensive cross-linking of proteins, which masks or blocks carbohydrate sites and prevents the formation of the reactive aldehydes needed for the Schiff reaction. Because of this masking effect, PAS staining is poor or negative with glutaraldehyde-fixed tissue. The other fixatives listed don’t mask those aldehyde sites to the same extent, so they are compatible with Schiff’s reagent, though some background or intensity differences may occur depending on the fixative.

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