Which enzyme would remove glycogen staining on histology slides?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

Which enzyme would remove glycogen staining on histology slides?

Explanation:
Glycogen detected by PAS staining can be selectively removed by digesting it with alpha-amylase (diastase). This enzyme breaks down glycogen, so after a diastase treatment the PAS stain attributed to glycogen disappears, confirming the material was glycogen. The other enzymes—lipase (lipids), protease (proteins), and nuclease (nucleic acids)—do not digest glycogen, so they won’t remove the glycogen-related PAS staining.

Glycogen detected by PAS staining can be selectively removed by digesting it with alpha-amylase (diastase). This enzyme breaks down glycogen, so after a diastase treatment the PAS stain attributed to glycogen disappears, confirming the material was glycogen. The other enzymes—lipase (lipids), protease (proteins), and nuclease (nucleic acids)—do not digest glycogen, so they won’t remove the glycogen-related PAS staining.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy