Which corrective action would fix a cover slip-related artifact described in the material?

• A. Remove the coverslip and reapply
• B. Apply more mounting medium
• C. Stain again
• D. Use a thicker section

Answer: (A)

Cover slip–related artifacts are usually due to trapped air, dust, or improper mounting between the tissue and the glass. Removing the coverslip and reapplying lets you clean the surface, replace any old or cloudy mounting medium, and reseal with fresh mounting medium so there are no air pockets or gaps. When you re-cover, place a small, even drop of mounting medium and lower the coverslip slowly at an angle to push out air, then wipe away excess medium and let it cure. Adding mounting medium without removing the coverslip can just trap more air or debris and won’t fix the underlying issue. Staining again or using a thicker section won’t address the physical artifact under the coverslip.