Which artifact is most likely responsible for lines observed in a microtomy section?

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Multiple Choice

Which artifact is most likely responsible for lines observed in a microtomy section?

Explanation:
When the blade edge is defective, the cut becomes rough and produces knife chatter, which shows up as lines in the microtomy section. A dull or nicked edge doesn’t shear tissue smoothly, so the blade and specimen vibrate slightly as they pass each other, creating tiny, parallel ridges across the surface. This knife-induced chatter is the classic source of fine lines seen in sections. In contrast, air bubbles would leave holes or voids, fixation artifacts alter staining and overall tissue morphology, and slippage tends to cause thickness or alignment issues rather than crisp line markings. Keeping the blade sharp, free of nicks, and properly aligned minimizes these lines.

When the blade edge is defective, the cut becomes rough and produces knife chatter, which shows up as lines in the microtomy section. A dull or nicked edge doesn’t shear tissue smoothly, so the blade and specimen vibrate slightly as they pass each other, creating tiny, parallel ridges across the surface. This knife-induced chatter is the classic source of fine lines seen in sections. In contrast, air bubbles would leave holes or voids, fixation artifacts alter staining and overall tissue morphology, and slippage tends to cause thickness or alignment issues rather than crisp line markings. Keeping the blade sharp, free of nicks, and properly aligned minimizes these lines.

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