When using malt diastase for glycogen digestion, what temperature guidance helps ensure complete digestion?

• A. Heat the solution to above 40°C to achieve complete digestion
• B. Cool the solution to 4°C
• C. Heat to 100°C
• D. Room temperature digestion only

Answer: (A)

Enzyme activity depends on temperature, and malt diastase works best at warm conditions. Heating the solution to a little above 40°C increases the enzyme’s catalytic rate enough to drive glycogen digestion toward completion within a practical time. Pushing to 100°C would denature the enzyme and stop digestion, while cooling to 4°C would slow or halt the reaction, and room temperature may not achieve complete digestion. So, heating to above 40°C is the guidance that ensures complete digestion.