What stain is used for Glycogen?

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Multiple Choice

What stain is used for Glycogen?

Explanation:
Glycogen detection uses a carbohydrate-staining method, with PAS (Periodic Acid–Schiff) staining highlighting glycogen as magenta. To specifically confirm glycogen, you treat the tissue first with diastase to digest glycogen, then apply PAS. If the substance was glycogen, the diastase digestion removes it and the subsequent PAS stain becomes negative. This diastase-PAS approach differentiates glycogen from other PAS-positive carbohydrates that resist diastase. The combination described—diastase digestion followed by PAS staining (Periodic Acid–Schiff reagent)—is the standard way to demonstrate glycogen.

Glycogen detection uses a carbohydrate-staining method, with PAS (Periodic Acid–Schiff) staining highlighting glycogen as magenta. To specifically confirm glycogen, you treat the tissue first with diastase to digest glycogen, then apply PAS. If the substance was glycogen, the diastase digestion removes it and the subsequent PAS stain becomes negative. This diastase-PAS approach differentiates glycogen from other PAS-positive carbohydrates that resist diastase. The combination described—diastase digestion followed by PAS staining (Periodic Acid–Schiff reagent)—is the standard way to demonstrate glycogen.

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