What stain is sometimes used after hyaluronidase digestion?

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Multiple Choice

What stain is sometimes used after hyaluronidase digestion?

Explanation:
Staining after enzyme digestion is used to differentiate types of mucopolysaccharides. Hyaluronidase digests hyaluronic acid, a non-sulfated glycosaminoglycan. After this digestion, applying Alcian Blue at pH 2.5 highlights the remaining acidic mucosubstances, specifically the sulfated ones, by turning them blue. This helps identify and characterize sulfated mucins in the tissue. The other stains don’t serve this post-digestion purpose: PAS targets a broad range of carbohydrates, Congo Red detects amyloid, and Hematoxylin is a general nuclear stain with no specific mucin differentiation after hyaluronidase treatment.

Staining after enzyme digestion is used to differentiate types of mucopolysaccharides. Hyaluronidase digests hyaluronic acid, a non-sulfated glycosaminoglycan. After this digestion, applying Alcian Blue at pH 2.5 highlights the remaining acidic mucosubstances, specifically the sulfated ones, by turning them blue. This helps identify and characterize sulfated mucins in the tissue. The other stains don’t serve this post-digestion purpose: PAS targets a broad range of carbohydrates, Congo Red detects amyloid, and Hematoxylin is a general nuclear stain with no specific mucin differentiation after hyaluronidase treatment.

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