What stain demos acid mucosubstances?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

What stain demos acid mucosubstances?

Explanation:
Acid mucosubstances are negatively charged mucopolysaccharides, such as sulfated and carboxylated glycosaminoglycans. Alcian Blue is a cationic dye that binds to those negative groups, so it stains acidic mucins blue. When used at pH 2.5, it detects both sulfated and carboxylated mucosubstances, making Alcian Blue the best choice to demonstrate acid mucosubstances. In contrast, PAS stains neutral mucopolysaccharides and mucins (magenta), Congo Red highlights amyloid, and Diastase is an enzyme used with PAS to digest glycogen rather than to stain acid mucosubstances.

Acid mucosubstances are negatively charged mucopolysaccharides, such as sulfated and carboxylated glycosaminoglycans. Alcian Blue is a cationic dye that binds to those negative groups, so it stains acidic mucins blue. When used at pH 2.5, it detects both sulfated and carboxylated mucosubstances, making Alcian Blue the best choice to demonstrate acid mucosubstances.

In contrast, PAS stains neutral mucopolysaccharides and mucins (magenta), Congo Red highlights amyloid, and Diastase is an enzyme used with PAS to digest glycogen rather than to stain acid mucosubstances.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy