What processing step uses vacuum to increase the rate of infiltration?

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Multiple Choice

What processing step uses vacuum to increase the rate of infiltration?

Explanation:
Vacuum-assisted infiltration speeds up how the embedding medium penetrates tissue. By reducing the ambient pressure, air trapped in tissue spaces is removed, which lowers the resistance to the embedding medium (usually molten paraffin) and lets it flow in more quickly and evenly. This improves infiltration, especially in dense or larger specimens, and can shorten processing time while reducing voids that cause poor sectioning. Other options serve different purposes: centrifugal force isn’t used to drive embedding medium into tissue, staining is for applying dyes, and clearing is a solvent step to replace water before infiltration.

Vacuum-assisted infiltration speeds up how the embedding medium penetrates tissue. By reducing the ambient pressure, air trapped in tissue spaces is removed, which lowers the resistance to the embedding medium (usually molten paraffin) and lets it flow in more quickly and evenly. This improves infiltration, especially in dense or larger specimens, and can shorten processing time while reducing voids that cause poor sectioning. Other options serve different purposes: centrifugal force isn’t used to drive embedding medium into tissue, staining is for applying dyes, and clearing is a solvent step to replace water before infiltration.

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