What is the maximum thickness allowed for a tissue specimen?

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Multiple Choice

What is the maximum thickness allowed for a tissue specimen?

Explanation:
Keeping tissue pieces thin ensures fixatives and processing reagents can reach all parts quickly and evenly. The maximum thickness used is 0.3 cm (about 3 millimeters). This size allows complete penetration during fixation and paraffin embedding, reducing centers that remain under-fixed and preventing artifacts in staining and histology sections. Pieces thicker than this risk incomplete fixation, which can lead to autolysis and uneven staining. So, 0.3 cm is the standard upper limit for processing. Smaller pieces (like 0.2 cm) also process well, while something as thick as 1.0 cm would not fix properly.

Keeping tissue pieces thin ensures fixatives and processing reagents can reach all parts quickly and evenly. The maximum thickness used is 0.3 cm (about 3 millimeters). This size allows complete penetration during fixation and paraffin embedding, reducing centers that remain under-fixed and preventing artifacts in staining and histology sections. Pieces thicker than this risk incomplete fixation, which can lead to autolysis and uneven staining. So, 0.3 cm is the standard upper limit for processing. Smaller pieces (like 0.2 cm) also process well, while something as thick as 1.0 cm would not fix properly.

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