What is the fixative for Phosphotungstic acid-hematoxylin?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

What is the fixative for Phosphotungstic acid-hematoxylin?

Explanation:
Phosphotungstic acid-hematoxylin staining depends on fixing the tissue in a way that preserves protein structure so the PTAH reaction can clearly differentiate muscle fibers and related structures. The fixatives that reliably support this stain are 10% neutral buffered formalin and Zenker’s fixative, because they maintain proteins without introducing artifactual changes that would mask the staining reaction. Osmium tetroxide is a lipid-preserving fixative used mainly for electron microscopy and isn’t used for PTAH. Bouin’s fixative is acidic and can alter staining patterns, making it less ideal for PTAH in many protocols. So, the fixatives that best support PTAH are 10% NBF and Zenker’s fixative.

Phosphotungstic acid-hematoxylin staining depends on fixing the tissue in a way that preserves protein structure so the PTAH reaction can clearly differentiate muscle fibers and related structures. The fixatives that reliably support this stain are 10% neutral buffered formalin and Zenker’s fixative, because they maintain proteins without introducing artifactual changes that would mask the staining reaction. Osmium tetroxide is a lipid-preserving fixative used mainly for electron microscopy and isn’t used for PTAH. Bouin’s fixative is acidic and can alter staining patterns, making it less ideal for PTAH in many protocols. So, the fixatives that best support PTAH are 10% NBF and Zenker’s fixative.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy