What is the fixative for Gomori reticulin?

• A. 10% NBF
• B. Formalin
• C. Bouin's fixative
• D. Alcohol

Answer: (A)

Fixing tissue for Gomori reticulin staining must preserve the delicate reticular network so the silver impregnation can clearly reveal it. Ten percent neutral buffered formalin provides stable, uniform fixation that maintains tissue architecture and reticular fibers without introducing reagents that interfere with the silver reaction. It preserves the chemical state of the fibers and keeps processing compatible with routine paraffin sections, giving crisp, reproducible reticulin staining. In contrast, Bouin's fixative, while historically used for its excellent preservation of fine structures, contains picric acid and can leave a yellow pigment and alter staining patterns, which can complicate the silver reaction. Alcohol fixatives tend to over-dehydrate and shrink tissues, which can degrade the reticular network and reduce staining quality. So, using 10% neutral buffered formalin is a reliable choice for Gomori reticulin.