What is a stated use of the Biotin-Avidin-Horseradish Peroxidase procedure?

• A. Surface marking of lymphomas
• B. Routine H&E staining
• C. In-situ hybridization
• D. Flow cytometry preparation

Answer: (A)

Biotin-avidin-horseradish peroxidase is an antibody-based detection method used in immunohistochemistry to show where specific antigens are located in tissue. The antibody binds to a target, and the biotin-avidin-HRP system amplifies that signal so you can see the exact spots under the microscope after adding a chromogen like DAB. This makes it especially useful for identifying surface antigens on lymphoid cells, which helps classify lymphomas by their immunophenotype. It’s not used for routine H&E staining, which relies on dyeing tissue components without antibody detection. It’s also not the standard approach for in-situ hybridization, which targets nucleic acids, nor is it a method used for preparing samples for flow cytometry, which analyzes cells in suspension rather than tissue sections.