True/False: Diastase and hyaluronidase are used to demo amyloid.

• A. True
• B. False
• C. Not sure
• D. Both True and False

Answer: (B)

Amyloid is demonstrated primarily with Congo red staining and looking for apple-green birefringence under polarized light. Enzymatic digestions like diastase (amylase) and hyaluronidase are used in other histochemical contexts—diastase to differentiate glycogen from other PAS-positive substances, and hyaluronidase to digest glycosaminoglycans and help distinguish mucopolysaccharide-rich materials. They do not form part of the routine demonstration of amyloid. So the statement is false.