Too long exposure in Verhoeff's Van Gieson staining yields elastic fibres that are which color?

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Multiple Choice

Too long exposure in Verhoeff's Van Gieson staining yields elastic fibres that are which color?

Explanation:
In Verhoeff's Van Gieson staining, elastic fibers are expected to stain black because the iron hematoxylin part of the stain binds strongly to them. If the staining time is too long, this intense black color can fade or shift as the dye diffuses and is partially washed out during differentiation steps. The result is that the elastic fibers no longer appear truly black and may look grey (not black) instead. The counterstain (Van Gieson) still colors surrounding collagen red and other tissue yellow, but the key change with overexposure is the loss of the characteristic black color in the elastic fibers.

In Verhoeff's Van Gieson staining, elastic fibers are expected to stain black because the iron hematoxylin part of the stain binds strongly to them. If the staining time is too long, this intense black color can fade or shift as the dye diffuses and is partially washed out during differentiation steps. The result is that the elastic fibers no longer appear truly black and may look grey (not black) instead. The counterstain (Van Gieson) still colors surrounding collagen red and other tissue yellow, but the key change with overexposure is the loss of the characteristic black color in the elastic fibers.

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