To remove AFH, what is the first step in the treatment sequence?

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Multiple Choice

To remove AFH, what is the first step in the treatment sequence?

Explanation:
The main idea is that removing AFH starts with dehydrating the tissue so solvent-based steps can work effectively. Bringing the section to absolute alcohol first fully removes water from the tissue, preparing it for subsequent AFH removal steps and preventing water from hindering solvent action. If you wait or move to an aqueous step, you’d reduce the effectiveness of AFH removal. Placing in saturated picric acid or washing with water comes after the initial dehydration, and proceeding directly to staining would trap any AFH and compromise results.

The main idea is that removing AFH starts with dehydrating the tissue so solvent-based steps can work effectively. Bringing the section to absolute alcohol first fully removes water from the tissue, preparing it for subsequent AFH removal steps and preventing water from hindering solvent action. If you wait or move to an aqueous step, you’d reduce the effectiveness of AFH removal. Placing in saturated picric acid or washing with water comes after the initial dehydration, and proceeding directly to staining would trap any AFH and compromise results.

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