To reduce the issue that cannot be totally eliminated, which practice is recommended?

• A. Changing deparaffinization solutions often
• B. Extending staining time
• C. Using higher temperature
• D. Reducing section thickness

Answer: (A)

Fresh changing of deparaffinization solutions is recommended because residual paraffin can always leave a hydrophobic film that interferes with dye penetration and produces artifacts. Replacing the solvents regularly keeps their ability to dissolve and remove paraffin high, so wax remnants and embedding medium are minimized. Extending staining time might worsen background or over-stain areas; higher temperature can damage tissue and increase nonspecific staining; reducing section thickness changes morphology but doesn’t address paraffin removal.