To link hematoxylin to tissue DNA what must be added?

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Multiple Choice

To link hematoxylin to tissue DNA what must be added?

Explanation:
Hematoxylin needs a mordant to form a dye-lake that can bind to tissue DNA. The mordant, usually an aluminum salt, links the oxidized form of hematoxylin (hematein) to the tissue, creating a complex that adheres to negatively charged nucleic acids in the nucleus. This is what makes nuclei stain a distinct blue/purple. A buffer helps keep the pH in the range where binding is optimal but does not create the link itself. Alcohol is for dehydration and can affect staining intensity, and salt influences ionic strength but does not establish the hematoxylin–DNA binding.

Hematoxylin needs a mordant to form a dye-lake that can bind to tissue DNA. The mordant, usually an aluminum salt, links the oxidized form of hematoxylin (hematein) to the tissue, creating a complex that adheres to negatively charged nucleic acids in the nucleus. This is what makes nuclei stain a distinct blue/purple. A buffer helps keep the pH in the range where binding is optimal but does not create the link itself. Alcohol is for dehydration and can affect staining intensity, and salt influences ionic strength but does not establish the hematoxylin–DNA binding.

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