Tissue must be washed in water after fixation in Zenker's. Which term is the fixative involved?

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Multiple Choice

Tissue must be washed in water after fixation in Zenker's. Which term is the fixative involved?

Explanation:
Zenker's fixative contains mercuric chloride along with chromates; its heavy-metal components can continue to interact with tissue and interfere with staining if not removed. Washing the tissue in water after fixation removes these residues, preventing artefacts and allowing proper stain uptake in later steps. This post-fixation washing is a hallmark of Zenker's fixation, so the fixative involved is Zenker's.

Zenker's fixative contains mercuric chloride along with chromates; its heavy-metal components can continue to interact with tissue and interfere with staining if not removed. Washing the tissue in water after fixation removes these residues, preventing artefacts and allowing proper stain uptake in later steps. This post-fixation washing is a hallmark of Zenker's fixation, so the fixative involved is Zenker's.

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