This staining method is useful to demonstrate both neutral and acidic lipids:

• A. Nile Blue Sulfate
• B. Oil Red O
• C. Sudan Black B
• D. OTAN

Answer: (A)

The staining method uses a dye that reveals lipid classes in two colors, so you can see both neutral and acidic lipids in the same section. Nile blue sulfate colors neutral lipids red-orange and acidic lipids blue, allowing you to distinguish the two lipid types within tissue. This dual-color distinction is especially useful on frozen sections where lipids are preserved, since standard paraffin processing dissolves them. Other stains mainly highlight one lipid type or don’t provide a clear two-color separation, making Nile blue sulfate the best choice for demonstrating both neutral and acidic lipids.