The recommended section thickness for crystal violet stains is 4-6 micrometers.

• A. True
• B. False
• C. 2-3 micrometers
• D. 8-10 micrometers

Answer: (B)

Crystal violet staining is used on slides and does not define a specific section thickness. In histology, the 4–6 micrometer range is a common thickness for paraffin-embedded tissue sections regardless of the stain used, not a property of crystal violet itself. If you’re staining bacteria on a smear (Gram stain), there’s no tissue section thickness to consider at all. So stating that the recommended section thickness for crystal violet stains is 4–6 μm is misleading—the thickness applies to prepared tissue sections in general, not to this stain specifically.