The PAS reaction with diastase digestion demonstrates the presence of which substance?

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Multiple Choice

The PAS reaction with diastase digestion demonstrates the presence of which substance?

Explanation:
Periodic acid–Schiff stains carbohydrates in tissues a magenta color. When you combine it with diastase digestion, you’re specifically testing for glycogen. Diastase (amylase) digests glycogen, so a structure that loses its PAS staining after diastase indicates that the original substance was glycogen. Structures like mucin or other diastase-resistant carbohydrates remain PAS-positive after digestion, so they are not glycogen. Therefore, the PAS reaction with diastase digestion demonstrates the presence of glycogen.

Periodic acid–Schiff stains carbohydrates in tissues a magenta color. When you combine it with diastase digestion, you’re specifically testing for glycogen. Diastase (amylase) digests glycogen, so a structure that loses its PAS staining after diastase indicates that the original substance was glycogen. Structures like mucin or other diastase-resistant carbohydrates remain PAS-positive after digestion, so they are not glycogen. Therefore, the PAS reaction with diastase digestion demonstrates the presence of glycogen.

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