The Feulgen reaction is unsatisfactory on tissue fixed in which fixative?

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Multiple Choice

The Feulgen reaction is unsatisfactory on tissue fixed in which fixative?

Explanation:
Feulgen staining detects DNA by converting deoxyribose to aldehyde groups with acid hydrolysis, which Schiff reagent then binds to, giving a magenta nuclear stain. The fixative used can alter DNA so that this aldehyde formation is hindered or masked. Bouin solution, which contains picric and acetic acids, can modify or extract nucleic acids and leave residues that interfere with the Schiff reaction, making the staining unreliable or weak. Because of this, tissue fixed in Bouin solution yields an unsatisfactory Feulgen result. Formalin, alcohol, and acetone fixatives preserve DNA in a way that allows proper hydrolysis and Schiff binding, producing the expected magenta nuclei.

Feulgen staining detects DNA by converting deoxyribose to aldehyde groups with acid hydrolysis, which Schiff reagent then binds to, giving a magenta nuclear stain. The fixative used can alter DNA so that this aldehyde formation is hindered or masked. Bouin solution, which contains picric and acetic acids, can modify or extract nucleic acids and leave residues that interfere with the Schiff reaction, making the staining unreliable or weak. Because of this, tissue fixed in Bouin solution yields an unsatisfactory Feulgen result. Formalin, alcohol, and acetone fixatives preserve DNA in a way that allows proper hydrolysis and Schiff binding, producing the expected magenta nuclei.

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