The Bodian technique fixative option among the following is which?

• A. NBF
• B. Bouin's
• C. Glutaraldehyde
• D. Alcohol

Answer: (A)

The Bodian nerve-fiber silver staining method requires a fixative that preserves fine tissue structure without introducing residues or excessive cross-linking that would interfere with silver impregnation and development. Neutral buffered formalin fits this need by fixing tissue gently enough to maintain morphology while avoiding the problematic residues and over-fixation associated with other fixatives. Bouin’s fixative contains picric acid, which can leave residues and cause background staining or interfere with silver deposition. Glutaraldehyde cross-links proteins heavily, which can hinder silver impregnation and reduce stain quality. Alcohol fixes dehydrate and shrink tissue, often leading to poor staining consistency. Therefore, neutral buffered formalin is the best match for the Bodian technique.