Sections in what fixative must be treated with iodine?

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Multiple Choice

Sections in what fixative must be treated with iodine?

Explanation:
Sections fixed in B-5 are treated with iodine because this fixative contains mercuric salts, and iodine helps neutralize or remove those mercury residues that can interfere with subsequent staining. The iodine step reduces background artifacts and improves stain uptake by clearing mercurial residues before dehydration and embedding. Other fixatives listed are either not mercury-based or use different demetallation steps, so they do not require this iodine treatment in routine processing.

Sections fixed in B-5 are treated with iodine because this fixative contains mercuric salts, and iodine helps neutralize or remove those mercury residues that can interfere with subsequent staining. The iodine step reduces background artifacts and improves stain uptake by clearing mercurial residues before dehydration and embedding. Other fixatives listed are either not mercury-based or use different demetallation steps, so they do not require this iodine treatment in routine processing.

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