PAS diastase digestion is a very sensitive histochemical method for which substance?

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Multiple Choice

PAS diastase digestion is a very sensitive histochemical method for which substance?

Explanation:
Periodic acid–Schiff stains carbohydrates by creating aldehyde groups that react with Schiff reagent to produce a magenta color. When diastase (amylase) digestion is performed before PAS (PAS-D), glycogen, a readily digestible glucose polymer, is hydrolyzed and removed. Other PAS-positive substances, like mucopolysaccharides and glycoproteins, are not digested by amylase and thus remain PAS-positive after diastase treatment. So, if the loss of PAS staining occurs after diastase, that indicates the original PAS positivity was due to glycogen. This selective digestion makes PAS diastase digestion a very sensitive method for detecting glycogen in tissues.

Periodic acid–Schiff stains carbohydrates by creating aldehyde groups that react with Schiff reagent to produce a magenta color. When diastase (amylase) digestion is performed before PAS (PAS-D), glycogen, a readily digestible glucose polymer, is hydrolyzed and removed. Other PAS-positive substances, like mucopolysaccharides and glycoproteins, are not digested by amylase and thus remain PAS-positive after diastase treatment. So, if the loss of PAS staining occurs after diastase, that indicates the original PAS positivity was due to glycogen. This selective digestion makes PAS diastase digestion a very sensitive method for detecting glycogen in tissues.

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