Once the issue is seen the only way to obtain a complete section is to which technique?

• A. Ribbon past the holes of tissue
• B. Re-embed and re-section
• C. Increase section thickness
• D. Use a different embedding medium

Answer: (A)

When you see holes in a tissue section, the practical way to get a complete section on a slide is to ribbon past the holes. By continuing to cut and collect a ribbon that extends beyond the defective area, tissue on either side of the void is captured in subsequent sections, increasing the chance of obtaining an intact, readable cross-section. Re-embedding and re-section could fix problems for future sections but doesn’t address the current defect in the ribbon; increasing section thickness reduces detail and can blur morphology; changing embedding medium won’t specifically fix holes caused by cutting artifacts.