Oil Red O technique shows what?

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Multiple Choice

Oil Red O technique shows what?

Explanation:
Oil Red O is a fat-specific dye that highlights neutral lipids, such as triglycerides and cholesteryl esters, staining them bright red in tissue sections. It is used on fresh or frozen tissue because routine paraffin processing removes lipids, so this staining preserves and reveals lipid droplets. This makes it the method of choice for demonstrating neutral fats, such as in adipose tissue or steatosis. It does not stain glycogen (which is shown with PAS), mucin (shown with Alcian blue or mucicarmine), or collagen (shown with Masson trichrome or Sirius red), hence neutral fats are the correct target for Oil Red O.

Oil Red O is a fat-specific dye that highlights neutral lipids, such as triglycerides and cholesteryl esters, staining them bright red in tissue sections. It is used on fresh or frozen tissue because routine paraffin processing removes lipids, so this staining preserves and reveals lipid droplets. This makes it the method of choice for demonstrating neutral fats, such as in adipose tissue or steatosis. It does not stain glycogen (which is shown with PAS), mucin (shown with Alcian blue or mucicarmine), or collagen (shown with Masson trichrome or Sirius red), hence neutral fats are the correct target for Oil Red O.

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