Oil Red O staining requires which type of sections?

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Multiple Choice

Oil Red O staining requires which type of sections?

Explanation:
Oil Red O is a lipid-specific stain, so it needs to visualize triglycerides and other neutral lipids that must stay in the tissue. Lipids are dissolved by the organic solvents used in standard paraffin or other plastic embedding processes, which would erase the lipids and prevent staining. Freezing the tissue preserves the lipids, and cutting unfixed or lightly fixed frozen sections (cryosections) keeps the lipid droplets intact for Oil Red O to bind and appear red. That’s why this stain is done on frozen sections rather than paraffin-embedded or other embedded preparations.

Oil Red O is a lipid-specific stain, so it needs to visualize triglycerides and other neutral lipids that must stay in the tissue. Lipids are dissolved by the organic solvents used in standard paraffin or other plastic embedding processes, which would erase the lipids and prevent staining. Freezing the tissue preserves the lipids, and cutting unfixed or lightly fixed frozen sections (cryosections) keeps the lipid droplets intact for Oil Red O to bind and appear red. That’s why this stain is done on frozen sections rather than paraffin-embedded or other embedded preparations.

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