Oil Red O Method is used to demonstrate what in tissue?

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Multiple Choice

Oil Red O Method is used to demonstrate what in tissue?

Explanation:
This question tests staining of lipids in tissue. Oil Red O is a fat‑soluble dye that specifically binds to neutral lipids, such as triglycerides and cholesterol esters, giving them a bright red–orange color. Because lipids are removed during routine paraffin processing, this stain is performed on frozen sections (or prepped before dehydration) to preserve lipid content. That makes Oil Red O the best choice for demonstrating neutral fats in tissue. It does not stain proteins, nucleic acids, or carbohydrates, which require different histochemical stains (proteins with protein stains, nucleic acids with techniques like Feulgen, carbohydrates with PAS, etc.).

This question tests staining of lipids in tissue. Oil Red O is a fat‑soluble dye that specifically binds to neutral lipids, such as triglycerides and cholesterol esters, giving them a bright red–orange color. Because lipids are removed during routine paraffin processing, this stain is performed on frozen sections (or prepped before dehydration) to preserve lipid content. That makes Oil Red O the best choice for demonstrating neutral fats in tissue. It does not stain proteins, nucleic acids, or carbohydrates, which require different histochemical stains (proteins with protein stains, nucleic acids with techniques like Feulgen, carbohydrates with PAS, etc.).

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