Nissl substance is demonstrated by which stain?

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Multiple Choice

Nissl substance is demonstrated by which stain?

Explanation:
Nissl substance, the rough endoplasmic reticulum and ribosomes in neurons, is basophilic because of its RNA content. To visualize it, you use a basic aniline dye that binds RNA; the classic choice is cresyl echt violet, which stains the Nissl bodies dark blue to purple and makes neuronal cell bodies stand out against the surrounding tissue. Luxol fast blue targets myelin lipids and is not used for highlighting Nissl substance. Hematoxylin does stain nuclei and other basophilic structures, but the clear, specific visualization of Nissl bodies is best with cresyl violet. Eosin stains cytoplasmic proteins pink and does not reveal the RNA-rich Nissl material.

Nissl substance, the rough endoplasmic reticulum and ribosomes in neurons, is basophilic because of its RNA content. To visualize it, you use a basic aniline dye that binds RNA; the classic choice is cresyl echt violet, which stains the Nissl bodies dark blue to purple and makes neuronal cell bodies stand out against the surrounding tissue. Luxol fast blue targets myelin lipids and is not used for highlighting Nissl substance. Hematoxylin does stain nuclei and other basophilic structures, but the clear, specific visualization of Nissl bodies is best with cresyl violet. Eosin stains cytoplasmic proteins pink and does not reveal the RNA-rich Nissl material.

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