In Verhoeff's Van Gieson staining, what color is cytoplasm?

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Multiple Choice

In Verhoeff's Van Gieson staining, what color is cytoplasm?

Explanation:
In Verhoeff's Van Gieson staining, the contrast comes from combining two staining steps: Verhoeff’s iron hematoxylin stains elastic fibers black, while the Van Gieson counterstain uses picric acid and acid fuchsin. Picric acid imparts a strong yellow color to cytoplasm and muscle fibers, whereas acid fuchsin stains collagen pink/red. This separation lets you distinguish tissue elements easily: elastic fibers appear black, cytoplasm yellow, and collagen pink/red. Therefore, the cytoplasm is yellow.

In Verhoeff's Van Gieson staining, the contrast comes from combining two staining steps: Verhoeff’s iron hematoxylin stains elastic fibers black, while the Van Gieson counterstain uses picric acid and acid fuchsin. Picric acid imparts a strong yellow color to cytoplasm and muscle fibers, whereas acid fuchsin stains collagen pink/red. This separation lets you distinguish tissue elements easily: elastic fibers appear black, cytoplasm yellow, and collagen pink/red. Therefore, the cytoplasm is yellow.

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