In Verhoeff's Van Gieson staining, what color are nuclei at the end of staining?

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Multiple Choice

In Verhoeff's Van Gieson staining, what color are nuclei at the end of staining?

Explanation:
Nuclei appear black because the iron hematoxylin component of Verhoeff’s stain binds to nuclear chromatin and yields a dark blue-black to black color. The Van Gieson counterstain then colors other tissue elements (like collagen pink/red and surrounding tissue yellow) but does not alter the nuclear staining. So, at the end, nuclei are seen as black.

Nuclei appear black because the iron hematoxylin component of Verhoeff’s stain binds to nuclear chromatin and yields a dark blue-black to black color. The Van Gieson counterstain then colors other tissue elements (like collagen pink/red and surrounding tissue yellow) but does not alter the nuclear staining. So, at the end, nuclei are seen as black.

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