In the processing schedule, which step uses heat?

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Multiple Choice

In the processing schedule, which step uses heat?

Explanation:
Heat is used to melt paraffin wax for tissue infiltration and embedding. After dehydration and clearing, the tissue is placed in molten paraffin (around 60°C) so the wax can permeate the tissue and support it for sectioning. Once infiltrated, the block is cooled to solidify. The alcohols and xylene are liquids used at room temperature (or mildly warmed) and do not require melting, so they do not involve heating in the same way.

Heat is used to melt paraffin wax for tissue infiltration and embedding. After dehydration and clearing, the tissue is placed in molten paraffin (around 60°C) so the wax can permeate the tissue and support it for sectioning. Once infiltrated, the block is cooled to solidify. The alcohols and xylene are liquids used at room temperature (or mildly warmed) and do not require melting, so they do not involve heating in the same way.

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